Summary: Following the completion of both a first and second mutagenicity study in accordance with OECD Test Guideline No. 471 at a GLP-certified testing facility, no cytotoxicity or mutagenicity was observed. Cytotoxicity testing was conducted at concentrations of 10, 100, 500, 1000, 2500, and 5000 µg per plate. Mutagenicity tests yielded negative results at concentrations of 50, 150, 500, 1500, and 5000 µg per plate in the first experiment (initially without metabolic activation, followed by testing with activation) and at 30, 100, 300, 1000, and 3000 µg per plate in the second experiment (conducted with pre-incubation, followed by testing with and without metabolic activation).
Categorisation according to Klimisch et al. 1997: "reliable without restriction"
The following price (net) is requested by the study owner: 60 000€ (incl. Test ID 702: 90-day subchronic toxicity study/CBD, Test ID 703: Comet Assay/CBD)
Summary: Two mutagenicity experiments were conducted based on the results of the cytotoxicity tests, following OECD Test Guideline No. 471 at a GLP-certified testing facility. No mutagenic effects were observed in any of the tested indicator strains, both with and without metabolic activation. The first experiment was performed at concentrations of 30, 100, 300, 1000, and 3000 µg per plate (with and without metabolic activation). The second experiment, which included metabolic activation and pre-incubation, used concentrations of 1, 3, 10, 30, and 100 µg per plate.
Categorisation according to Klimisch et al. 1997: "reliable without restriction"
The following price (net) is requested by the study owner: 2 500€